[ Herbal Remedies ]

NETosis Delays Diabetic Wound Healing in Mice and Humans. – PubMed


UNIVERSITY PARK, Pa. Diabetes and ischemia are two major risk factors behind impaired healing of chronic lower extremity wounds. In this paper, we seek to determine whether topical application of H2O2 can modulate wound healing and if its effects are related to oxidative damage. Using electrophoretic mobility shift assays of wound tissue from IL-6 KO mice, decreased AP-1 transcription factor activation was shown compared with wild-type mice 16 h after wounding. The research was performed by scientists at the Departments of Dermatology and Ophthalmology of Kyoto Prefectural University School of Medicine, Kyoto, Japan; the Department of Dermatology at Hamamatsu University School of Medicine, Hamamatsu, Japan; and Shiseido Innovative Scientific Research Center, Yamamoto, Japan. Diabetes was induced by multiple low doses of streptozotocin in bioengineered human-skin-engrafted immunodeficient mice. Moreover, db/db of laser irradiation was compared with db/db of non laser irradiation, finding capillary dilatation and regeneration, the proliferation of fibroblasts and collagen fibers prominent.

If a majority of non-healing wounds contain biofilm, and it has a role in delayed healing, then biofilm could be contributing many billions of dollars to the global cost of chronic wounds.[20,21] Recent evidence from animal models has demonstrated that biofilm creates a low-grade and persistent inflammatory response, and impairs both epithelialization and granulation tissue formation.[22] Additionally, due to clinical observations of suspected biofilm, specific biofilm-based wound management is now being practised.[12] This review will focus on the most recent clinical experiences and in vivo evidence from relevant animal models to summarize the latest knowledge of the effects of biofilm on wound healing. Lastly, there appeared to be higher levels of apoptosis in diabetic wounds, shown by the terminal transferase mediated UTP nick end-labelling technique. Healing of splinted wounds was delayed compared to unsplinted wounds, but laser irradiation (1.6 J/day, 7 days) stimulated healing by re-epithelization and granulation tissue formation. They discovered that high dose aspirin, the most commonly used nonsteroidal anti-inflammatory drug, delays wound healing by reducing the production of 12-HHT. Diabetes is associated with increased morbidity and mortality,1 as well as impaired wound healing.2 Diabetes-related admissions accounted for 22% of all hospital inpatient days in 2007, and diabetic foot ulcers account for 20% of all hospital admissions in diabetic patients, which are the leading cause of lower extremity amputations.2 Improvements made in diabetic wound management and prevention clearly have the potential to affect a large number of patients and decrease diabetic-related health care expenditures. © 2016 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.

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