[ Diabetes Type 2 ]

Amylase Activity and Its Isoenzyme Analysis in Juvenile-Onset Diabetes Mellitus

Objectives: This report from the LEADER (Liraglutide Effect and Action in Diabetes: Evaluation of Cardiovascular Outcome Results) trial describes baseline lipase and amylase activity in type 2 diabetic subjects without acute pancreatitis symptoms before randomization to the glucagonlike peptide analog liraglutide or placebo. The rest comes from your salivary glands. However, all of these treatments have limited efficacy and are associated with undesirable side effects [1–3], leading to increasing interest in the use of medicinal plants for the alternative management of type 2 diabetes mellitus. Sahendica is one of the endemic species, which was already subjected to phytochemical investigation on both extract and essential and predicted to exhibit anti-diabetic activity due to the presence of beta-sitosterol[14],[16],[17]. Dotriacontanyl docosanoate and the mixture of oleanolic acid and ursolic acid are reported from this plant species for the first time. Glycosidic linkages of α-D-(1,4) in carbohydrates are cleaved by α-amylase to produce oligosaccharides, which are further cleaved to monosaccharide glucose by α-glucosidase [3]. Materials and Methods: A total of 40 individuals, 20 with Type 2 diabetes and 20 controls of age group 40–60 years were selected for the study.

Injections of these diabetic rats with insulin increased pancreatic amylase mRNA contents significantly in both young and old rats. By contrast, light to moderate insulin resistance is compensated for by increased insulin secretion, or moderately increased insulin secretion does not accompany insulin resistance, both of which lead to increased insulinotropic action and possibly increased serum amylase. The following results were obtained. In both diabetic and normal groups in all doses nearly dose dependent manner reduced blood glucose and insulin levels. 2) The P-Am in JOD was significantly lower than that in so-called MODY. Especially in childhood-onset diabetes among JOD, the P-Am was particularly low and in some cases was undetectable. 3) The serum P-Am was significantly correlated with ΣCPR.

4) The serum T-Am was significantly increased after treatment of the diabetes, but the amylase clearance remained unchanged. In the isoenzyme study, the P-Am in JOD was not increased after treatment, although in so-called MODY both the T-Am and P-Am were increased in parallel. Another focus will involve identifying the neuroendocrine mechanisms that connect starch breakdown in the mouth with insulin release.

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